Efecto del extracto liofilizado de Juglans neotropica Diels sobre la ansiedad, depresión y memoria inducida por separación materna en Rattus norvegicus var. Holtzman
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente trabajo tuvo como objetivo evaluar el efecto del extracto liofilizado de Juglans neotropica Diels sobre la ansiedad, depresión y memoria inducida por separación materna en Rattus norvegicus var. Holtzman. El material botánico fue recolectado del distrito de Chimbote, provincia del santa, región de Ancash, Perú, se estabilizaron a -70ºC y luego fueron liofilizados. Se utilizaron 24 especímenes de Rattus norvegicus var. Holtzman, divididos en 2 grupos, problema (fueron sometidos a separación materna por 4 horas durante 14 días consecutivos) y control negativo. Para la obtención de los datos basales se esperó que los especímenes adquieran la edad adulta (2 meses). Se utilizaron 4 modelos farmacológicos: laberinto en cruz elevado y campo abierto (open field) para determinar ansiedad, nado forzado de Porsolt para determinar depresión y test de Morris para luego determinar la alteración negativa sobre la memoria consecuente del estrés inducido por separación materna. Posterior a ello, el grupo Problema se subdividió en 5 grupos, Problema 1 (0.5g/Kg) y Problema 2 (1g/Kg) a los cuales se les administró el extracto liofilizado de Juglans neotropica Diels durante 14 días; Patron 1 (bromazepam 10mg/mL durante 14 dias), Patron 2 (fluoxetina 1mg/mL durante 14 días) y control positivo (solución salina fisiológica). Por último, se realizaron nuevamente los mismos modelos farmacológicos para obtener los datos post tratamiento. Los datos se analizaron mediante la prueba de ANOVA y Test de Tukey como prueba post hoc. Los resultados evidenciaron que las dosis 0.5g/Kg y 1g/Kg del extracto liofilizado de Juglans neotropica Diels presentaron similar efecto que bromazepam en laberinto en cruz elevado y Open Field como efecto ansiolítico; así mismo presentaron similar efecto que fluoxetina en nado forzado de Porsolt como efecto antidepresivo, sin embargo, como efecto sobre el deterioro de memoria, no se obtuvieron resultados marcados positivos con las dosis del extracto del liofilizado. Se concluye que Juglans neotropica Diels en dosis de 0.5g/Kg y 1g/Kg tiene efecto antidepresivo, ansiolítico en modelo animal y se necesitan más estudios para corroborar el efecto sobre el deterioro de memoria.
ABSTRACT The objective of this study was to evaluate the effect of the freeze-dried extract of Juglans neotropica Diels on anxiety, depression and memory induced by maternal separation in Rattus norvegicus var. Holtzman. The botanical material was collected from the district of Chimbote, province of Santa, Ancash region, Peru, stabilized at -70ºC and then freeze- dried. 24 specimens of Rattus norvegicus var. Holtzman were used, divided into 2 groups, problem (subjected to maternal separation for 4 hours during 14 consecutive days) and negative control. To obtain the baseline data, the specimens were expected to reach adulthood (2 months). Four pharmacological models were used: elevated plus maze and open field to determine anxiety, Porsolt forced swim to determine depression and Morris test to later determine the negative alteration on memory resulting from the stress induced by maternal separation. After that, the Problem group was subdivided into 5 groups, Problem 1 (0.5 g / Kg) and Problem 2 (1 g / Kg) to which the lyophilized extract of Juglans neotropica Diels was administered for 14 days; Pattern 1 (bromazepam 10 mg / mL for 14 days), Pattern 2 (fluoxetine 1 mg / mL for 14 days) and positive control (physiological saline solution). Finally, the same pharmacological models were performed again to obtain the post-treatment data. The data were analyzed using the ANOVA test and Tukey's test as a post hoc test. The results showed that the 0.5 g / Kg and 1 g / Kg doses of the lyophilized extract of Juglans neotropica Diels presented a similar effect to bromazepam in the elevated plus maze and Open Field as an anxiolytic effect; Likewise, they presented a similar effect to fluoxetine in Porsolt forced swimming as an antidepressant effect, however, as an effect on memory impairment, no marked positive results were obtained with the doses of the lyophilized extract. It is concluded that Juglans neotropica Diels in doses of 0.5 g/kg and 1 g/kg has an antidepressant, anxiolytic effect in animal models and more studies are needed to corroborate the improvement effect on memory impairment.
ABSTRACT The objective of this study was to evaluate the effect of the freeze-dried extract of Juglans neotropica Diels on anxiety, depression and memory induced by maternal separation in Rattus norvegicus var. Holtzman. The botanical material was collected from the district of Chimbote, province of Santa, Ancash region, Peru, stabilized at -70ºC and then freeze- dried. 24 specimens of Rattus norvegicus var. Holtzman were used, divided into 2 groups, problem (subjected to maternal separation for 4 hours during 14 consecutive days) and negative control. To obtain the baseline data, the specimens were expected to reach adulthood (2 months). Four pharmacological models were used: elevated plus maze and open field to determine anxiety, Porsolt forced swim to determine depression and Morris test to later determine the negative alteration on memory resulting from the stress induced by maternal separation. After that, the Problem group was subdivided into 5 groups, Problem 1 (0.5 g / Kg) and Problem 2 (1 g / Kg) to which the lyophilized extract of Juglans neotropica Diels was administered for 14 days; Pattern 1 (bromazepam 10 mg / mL for 14 days), Pattern 2 (fluoxetine 1 mg / mL for 14 days) and positive control (physiological saline solution). Finally, the same pharmacological models were performed again to obtain the post-treatment data. The data were analyzed using the ANOVA test and Tukey's test as a post hoc test. The results showed that the 0.5 g / Kg and 1 g / Kg doses of the lyophilized extract of Juglans neotropica Diels presented a similar effect to bromazepam in the elevated plus maze and Open Field as an anxiolytic effect; Likewise, they presented a similar effect to fluoxetine in Porsolt forced swimming as an antidepressant effect, however, as an effect on memory impairment, no marked positive results were obtained with the doses of the lyophilized extract. It is concluded that Juglans neotropica Diels in doses of 0.5 g/kg and 1 g/kg has an antidepressant, anxiolytic effect in animal models and more studies are needed to corroborate the improvement effect on memory impairment.
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Juglans neotropica Diels. Ansiedad. Depresión. Deterioro de memoria. Llaberinto en cruz elevado. Open Field. Nado Forzado de Porsolt y test de Morris