Actividad antifúngica del extracto etanólico de Ipomoea batatas L. “camote morado” frente a Candida albicans
No Thumbnail Available
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
Debido al uso continuo de fármacos empleados para tratar candidiasis y a sus frecuentes efectos adversos, es que este trabajo de investigación tiene como objetivo evaluar la actividad antifúngica del extracto etanólico de Ipomoea batatas L. “camote morado” frente a Candida albicans, a fin de establecer el potencial antifúngico de Ipomoea batatas L. mediante la concentración inhibitoria mínima (CIM). La planta de Ipomoea batatas Lam “camote morado” fue adquirido en el centro poblado de Mochal, en el distrito de Poroto, Provincia de Trujillo, Región La Libertad, a partir de las cuales se obtuvo el extracto etanólico agregando alcohol de 96° acidificado a pH 3,5 a las cáscaras del camote morado, se maceró durante 7 días con agitación manual diaria. La evaluación de la actividad antifúngica se llevó a cabo mediante el método de microdilución en placa colorimétrico, usando resazurina 0,1 % por pozo, como indicador de lectura, fue excelente para la evaluación de la susceptibilidad antifúngica contra Candida albicans. Los resultados evidencian que la cáscara de camote morado posee actividad antifúngica esto se ve evidenciado al hacer microdiluciones y al aplicarle la rezarsurina pudimos observar que el camote morado inhibió el crecimiento de la Candida albicans cambiando de fucsia a morado y evidenciando de esta manera la muerte de Candida albicans. Concluyendo que el extracto etanólico de Ipomoea batatas L. posee actividad antifúngica frente a Candida albicans.
ABSTRACT Owing to the continuous use of drugs used to treat candidiasis and their frequent adverse effects, this research work aims to evaluate the antifungal activity of the ethanolic extract of Ipomoea batatas L. "purple sweet potato" against Candida albicans, in order to establish the antifungal potential of Ipomoea batatas L. "purple sweet potato" by means of the minimum inhibitory concentration (MIC). The Ipomoea batatas Lam "sweet potato" plant was acquired in the town of Mochal, in the district of Poroto, Trujillo Province, La Libertad Region, from which the ethanolic extract was obtained by adding acidified 96 ° alcohol to pH 3.5 to purple sweet potato skins, macerated for 7 days with daily manual stirring. The evaluation of the antifungal activity was carried out by the microdilution method in a colorimetric plate, using resazurin 0,1 % as a reading indicator, it was excellent for the evaluation of the antifungal susceptibility against Candida albicans. The results show that the purple sweet potato peel has antifungal activity, this is evidenced by making microdilutions and by applying rezarsurin to it, we were able to show that the purple sweet potato inhibited the growth of Candida albicans, changing from pink to purple color and thus evidencing death. by Candida albicans. Concluding that the ethanolic extract of Ipomoea batatas L. "purple sweet potato" has antifungal activity against Candida albicans.
ABSTRACT Owing to the continuous use of drugs used to treat candidiasis and their frequent adverse effects, this research work aims to evaluate the antifungal activity of the ethanolic extract of Ipomoea batatas L. "purple sweet potato" against Candida albicans, in order to establish the antifungal potential of Ipomoea batatas L. "purple sweet potato" by means of the minimum inhibitory concentration (MIC). The Ipomoea batatas Lam "sweet potato" plant was acquired in the town of Mochal, in the district of Poroto, Trujillo Province, La Libertad Region, from which the ethanolic extract was obtained by adding acidified 96 ° alcohol to pH 3.5 to purple sweet potato skins, macerated for 7 days with daily manual stirring. The evaluation of the antifungal activity was carried out by the microdilution method in a colorimetric plate, using resazurin 0,1 % as a reading indicator, it was excellent for the evaluation of the antifungal susceptibility against Candida albicans. The results show that the purple sweet potato peel has antifungal activity, this is evidenced by making microdilutions and by applying rezarsurin to it, we were able to show that the purple sweet potato inhibited the growth of Candida albicans, changing from pink to purple color and thus evidencing death. by Candida albicans. Concluding that the ethanolic extract of Ipomoea batatas L. "purple sweet potato" has antifungal activity against Candida albicans.
Description
Keywords
Candidiasis, plantas medicinales, camote, Ipomoea batata L.