Efecto del liofilizado de Vaccinium corymbosum L. en depresión experimental, en modelo animal
No Thumbnail Available
Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
El presente trabajo tuvo como objetivo determinar el efecto que posee el liofilizado de Vaccinium corymbosum L(VC). en un modelo animal, en depresión experimental. Los frutos de VC, fueron recolectados de la provincia de Virú, región La Libertad, Perú, se estabilizaron a -80 °C y luego fueron liofilizados. Se utilizaron 24 especímenes machos de Rattus norvegicus var Holtzman, divididos en 4 grupos, control (solución salina fisiológica), patrón (fluoxetina 20 mg/kg) y 2 grupos problema (liofilizado del fruto de Vaccinium corymbosum L. de 10mg/kg y 20 mg/kg), durante 28 días. Se utilizó el método del Test de nado forzado modificado o Test de Porsolt modificado para determinación de la actividad antidepresiva. Las variables evaluadas fueron inmovilidad, nado horizontal y nado vertical durante 5 minutos en el último día del tratamiento. Los datos se analizaron mediante la prueba de ANOVA y test de Bonferroni como prueba post hoc. Los resultados en nado horizontal (swimming) e inmovilidad evidenciaron que las dosis de 10 y 20 mg/kg del liofilizado de arándano presentaron similar efecto que fluoxetina. Se concluye que VC, en dosis de 10 y 20 mg/kg tienen efecto antidepresivo en modelo animal.
ABSTRACT The aim of this study was to determine the effect of freeze-dried Vaccinium corymbosum L(VC) on an animal model in experimental depression. VC fruits were collected from the province of Viru, La Libertad region, Peru, stabilised at -80 °C and then freeze-dried. Twenty-four male specimens of Rattus norvegicus var Holtzman were divided into four groups, control (physiological saline solution), standard (fluoxetine 20 mg/kg) and two problem groups (freeze-dried Vaccinium corymbosum L. fruit 10 mg/kg and 20 mg/kg), for 28 days. The modified forced swim test or modified Porsolt test method was used to determine antidepressant activity. The variables assessed were immobility, horizontal swimming and vertical swimming for 5 minutes on the last day of treatment. Data were analysed using ANOVA and Bonferroni test as a post hoc test. The results on horizontal swimming and immobility showed that the 10 and 20 mg/kg doses of freeze-dried cranberry had a similar effect to fluoxetine. It is concluded that VC, at doses of 10 and 20 mg/kg, have an antidepressant effect in animal models.
ABSTRACT The aim of this study was to determine the effect of freeze-dried Vaccinium corymbosum L(VC) on an animal model in experimental depression. VC fruits were collected from the province of Viru, La Libertad region, Peru, stabilised at -80 °C and then freeze-dried. Twenty-four male specimens of Rattus norvegicus var Holtzman were divided into four groups, control (physiological saline solution), standard (fluoxetine 20 mg/kg) and two problem groups (freeze-dried Vaccinium corymbosum L. fruit 10 mg/kg and 20 mg/kg), for 28 days. The modified forced swim test or modified Porsolt test method was used to determine antidepressant activity. The variables assessed were immobility, horizontal swimming and vertical swimming for 5 minutes on the last day of treatment. Data were analysed using ANOVA and Bonferroni test as a post hoc test. The results on horizontal swimming and immobility showed that the 10 and 20 mg/kg doses of freeze-dried cranberry had a similar effect to fluoxetine. It is concluded that VC, at doses of 10 and 20 mg/kg, have an antidepressant effect in animal models.
Description
Keywords
Vaccinium corymbosum L., Depresión, Nado forzado.