Cuantificación de hierro presente en hígado de Capra aegagrus hircus “cabrito” mediante el método de Munsey.
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Date
2021
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Universidad Nacional de Trujillo
Abstract
En el presente trabajo se determinó la concentración de hierro en hígado de Capra aegagrus hircus, usando el método colorimétrico de Munsey. Se procedió con la preparación de las muestras, las cuales se homogenizaron, luego se llevaron a secar en la estufa hasta peso constante, después se llevaron a la mufla hasta la obtención de cenizas blancas, una vez realizado esto se analizaron las muestras examen. Se elaboró una curva de calibración estándar, el cual se tuvo como sustancia patrón al Amonio y hierro (II) sulfato hexahidratado, finalmente se realizó las lecturas en el espectrofotómetro a 550 nm. Se encontró que la concentración de hierro por 100g de muestra de hígado de Capra aegagrus hircus fue de 7,79 mg.
ABSTRACT In the present work, the iron concentration in the liver of Capra aegagrus hircus was determined, using the Munsey colorimetric method. We proceeded with the preparation of the samples, which were homogenized, then they were dried in the oven until constant weight, then they were placed in the muffle until the white ash was obtained. Once this was done, the test samples were analyzed. A standard calibration curve was elaborated, which had as standard substance Ammonium and iron (II) sulfate hexahydrate, finally the readings were made in the spectrophotometer at 550 nm. The iron concentration per 100g of Capra aegagrus hircus liver sample was found to be 7.79 mg.
ABSTRACT In the present work, the iron concentration in the liver of Capra aegagrus hircus was determined, using the Munsey colorimetric method. We proceeded with the preparation of the samples, which were homogenized, then they were dried in the oven until constant weight, then they were placed in the muffle until the white ash was obtained. Once this was done, the test samples were analyzed. A standard calibration curve was elaborated, which had as standard substance Ammonium and iron (II) sulfate hexahydrate, finally the readings were made in the spectrophotometer at 550 nm. The iron concentration per 100g of Capra aegagrus hircus liver sample was found to be 7.79 mg.
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Keywords
Aegagrus Capra hircus. Hierro.