Efecto de una pomada del extracto etanólico de Desmodium molliculum sobre quemadura de piel inducida en Mus musculus
No Thumbnail Available
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
El objetivo de esta investigación fue determinar el efecto cicatrizante del extracto etanólico de Desmodium molliculum en quemaduras de 2do grado inducidas en Mus musculus BALB/c. Se formaron 5 grupos experimentales de 9 individuos macho cada uno: Control Negativo (GCN) sin tratamiento, Control Farmacológico (GCF) tratado con sulfadiazina de plata al 1% en crema, Grupo Pomada Base (GPB) tratado con la formulación de la pomada base, Grupo Experimental 1% (DM1) tratado con pomada a base de Desmodium molliculum al 1% y el Grupo experimental 2% (DM2) tratado con pomada a base de Desmodium molliculum al 2%. Se midió el diámetro de la quemadura durante 21 días usando un vernier y se realizó análisis histopatológico; los resultados fueron sometidos a prueba ANOVA y Tukey, ambas con un 95% de confianza. Al día 15 la cicatrización fue total en los grupos DM1 y DM2 (ambos 0,00 ± 0,00 mm), mientras que los grupos GCN (1,30 ± 0,01 mm), GPB (0,70 ± 0,01 mm) y GCF (0,20 ±0,01 mm) aún presentaron heridas. Se concluyó que las dosis de 1% y 2% del extracto etanólico de Desmodium molliculum presentan efecto cicatrizante similar, disminuyen el tiempo de cicatrización y aumentan la regeneración celular, lo cual fue corroborado con la histopatología de la piel afectada.
The objective of this research was to determine the healing effect of the ethanolic extract of Desmodium molliculum on 2nd degree burns induced in Mus musculus BALB/c. 5 experimental groups of 9 male individuals each were formed: Negative Control (GCN) without treatment, Pharmacological Control (GCF) treated with 1% silver sulfadiazine cream, Base Ointment Group (GPB) treated with the base ointment formulation. , Experimental Group 1% (DM1) treated with Desmodium molliculum-based ointment at 1% and Experimental Group 2% (DM2) treated with Desmodium molliculum-based ointment at 2%. Burn diameter was measured for 21 days using a vernier and histopathological analysis was performed; the results were subjected to ANOVA and Tukey tests, both with 95% confidence. On day 15, healing is complete in the DM1 and DM2 groups (both 0.00 ± 0.00 mm), while the GCN (1.30 ± 0.01 mm), GPB (0.70 ± 0.01 mm) and GCF (0.20 ±0.01 mm) still have wounds. It was concluded that the doses of 1% and 2% of the ethanolic extract of Desmodium molliculum have a similar healing effect, decrease healing time and increase cell regeneration, which was corroborated with the histopathology of the affected skin.
The objective of this research was to determine the healing effect of the ethanolic extract of Desmodium molliculum on 2nd degree burns induced in Mus musculus BALB/c. 5 experimental groups of 9 male individuals each were formed: Negative Control (GCN) without treatment, Pharmacological Control (GCF) treated with 1% silver sulfadiazine cream, Base Ointment Group (GPB) treated with the base ointment formulation. , Experimental Group 1% (DM1) treated with Desmodium molliculum-based ointment at 1% and Experimental Group 2% (DM2) treated with Desmodium molliculum-based ointment at 2%. Burn diameter was measured for 21 days using a vernier and histopathological analysis was performed; the results were subjected to ANOVA and Tukey tests, both with 95% confidence. On day 15, healing is complete in the DM1 and DM2 groups (both 0.00 ± 0.00 mm), while the GCN (1.30 ± 0.01 mm), GPB (0.70 ± 0.01 mm) and GCF (0.20 ±0.01 mm) still have wounds. It was concluded that the doses of 1% and 2% of the ethanolic extract of Desmodium molliculum have a similar healing effect, decrease healing time and increase cell regeneration, which was corroborated with the histopathology of the affected skin.
Description
Keywords
Desmodium molliculum, mashua, regeneración celular, cicatrización, histopatología