Aislamiento y Caracterización de Bacteriófagos para Escherichia coli Multirresistentes, en muestras de pacientes de la ciudad de Huaraz – 2022.
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Date
2023
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Universidad Nacional de Trujillo
Abstract
Los bacteriófagos son virus que infectan bacterias, incluyendo bacterias patógenas como la Escherichia coli, una bacteria ubicua que también se le puede localizar en el microbiota natural del ser humano y/o causando daños y patologías, como en el caso de las infecciones a las vías urinarias, tanto en varones como en mujeres. Frente a ello los bacteriófagos son una posibilidad de solución para reducir o eliminar esta bacteria patógena que en muchos casos va siendo muy resistente a diferentes antibióticos y convirtiéndose con el tiempo en un serio problema de salud pública. En el presente estudio se aislaron seis bacterias de Escherichia coli resistentes a antibióticos provenientes de pacientes con urocultivos positivos, y aislamos tres tipos de bacteriófagos provenientes de aguas servidas del desagüe del Laboratorio clínico Biocells Dx del cual se aisló el fago tres; y del desagüe del Hospital II Essalud Huaraz de cual aislamos los Fagos -1 y Fagos-2. La caracterización de los fagos se realizó mediante Temperatura, pH y Luz UV. Así mismo se realizó el rango de hospedante con los seis cultivos bacterianos de Eschericia coli que logramos encontrar y enfrentarlos a los tres bacteriófagos que se logró aislar y purificar. El presente trabajo es una investigación básica que puede servir como base para futuros trabajos al respecto, debido a que cada área geográfica en donde se ubican las diferentes bacterias tiene sus propias peculiaridades y pese a ser la misma bacteria patógena, ésta se comporta de diferente manera.
Bacteriophages are viruses that infect bacteria, including pathogenic bacteria such as Escherichia coli, a ubiquitous bacterium that can also be found in the natural microbiota of humans and/or cause damage and pathologies, as in the case of airway infections. Urinary tract in both men and women. Faced with this, bacteriophages are a possible solution to reduce or eliminate this pathogenic bacterium, which in many cases is highly resistant to different antibiotics and, over time, becomes a serious public health problem. In the present study, six antibiotic-resistant Escherichia coli bacteria were isolated from patients with positive urine cultures, and we isolated three types of bacteriophages from sewage drains from the Biocells Dx Clinical Laboratory from which phage three was isolated; and from the drain of Hospital II Essalud Huaraz from which we isolated Phages -1 and Phages-2. The characterization of the phages was carried out by means of Temperature, Ph and UV Light. Likewise, the host range was carried out with the six Eschericia coli strains that we were able to find and confront them with the three bacteriophages that were isolated and purified. The present work is a basic investigation that can serve as a basis for future work in this regard, because each geographical area where the different bacteria are located has its own peculiarities and despite being the same pathogenic bacterium, it behaves differently.
Bacteriophages are viruses that infect bacteria, including pathogenic bacteria such as Escherichia coli, a ubiquitous bacterium that can also be found in the natural microbiota of humans and/or cause damage and pathologies, as in the case of airway infections. Urinary tract in both men and women. Faced with this, bacteriophages are a possible solution to reduce or eliminate this pathogenic bacterium, which in many cases is highly resistant to different antibiotics and, over time, becomes a serious public health problem. In the present study, six antibiotic-resistant Escherichia coli bacteria were isolated from patients with positive urine cultures, and we isolated three types of bacteriophages from sewage drains from the Biocells Dx Clinical Laboratory from which phage three was isolated; and from the drain of Hospital II Essalud Huaraz from which we isolated Phages -1 and Phages-2. The characterization of the phages was carried out by means of Temperature, Ph and UV Light. Likewise, the host range was carried out with the six Eschericia coli strains that we were able to find and confront them with the three bacteriophages that were isolated and purified. The present work is a basic investigation that can serve as a basis for future work in this regard, because each geographical area where the different bacteria are located has its own peculiarities and despite being the same pathogenic bacterium, it behaves differently.
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Keywords
Eschericia coli, bacteriófago, patologías, pH, Luz UV, rango de hospedante