Efecto de la concentración del aceite esencial de Cymbopogon citratus sobre el crecimiento de Microsporum canis y Trichophyton rubrum in vitro.
No Thumbnail Available
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
Se evaluó el efecto de la concentración del aceite esencial de Cymbopogon citratus_x000D_
sobre el crecimiento de Microsporum canis y Trichophyton rubrum in vitro. Para lo_x000D_
cual, se preparó Agar Sabouraud conteniendo concentraciones de dicho aceite_x000D_
esencial de 0.1, 0.5, 1, 1.5 y 2 μL/mL, respectivamente y además del control (0_x000D_
μL/mL). Sobre dicho medio se sembró por puntura central un fragmento de micelio_x000D_
de dichos hongos en estudio, provenientes de un cultivo monospórico, se incubó a_x000D_
25 °C hasta 14 días. Se realizó tres repeticiones por triplicado por cada_x000D_
concentración incluyendo el control. La lectura del crecimiento micelial se realizó_x000D_
midiendo el diámetro de la colonia en milímetros a partir del séptimo día de siembra_x000D_
hasta el décimo cuarto día, se encontró que el aceite esencial de hierbaluisa a las_x000D_
concentraciones de 0.1, 0.5, 1, 1.5 y 2 μL/mL inhiben completamente el crecimiento_x000D_
de los hongos en estudio. Concluyéndose que el aceite esencial es un fuerte_x000D_
inhibidor del crecimiento de M. canis y T. rubrum.
Description
This thesis work had as purpose to evaluate the effect of essential oil of_x000D_
Cymbopogon citratus. “lemongrass” on the growth of Microsporum canis and_x000D_
Trichophyton rubrum in vitro. For which, I prepared Sabouraud Agar containing_x000D_
concentrations of the said essential oil, 0.1, 0.5, 1, 1.5, and 2 μL/mL, respectively,_x000D_
and in addition to the control (0 μL/mL). On this medium was inoculated by puncture_x000D_
central a fragment of mycelium of these fungi in the study, coming from a culture_x000D_
monosporic, was incubated at 25 °C up to 14 days. We performed three replicates_x000D_
in triplicate for each concentration including the control. The reading of mycelial_x000D_
growth was performed in millimeters the diameter from the seventh day of sowing_x000D_
until the fourteenth day, it was found that the essential oil of lemongrass from the_x000D_
concentration including the control._x000D_
The mycelial growth was measured by measuring the diameter of the colony in_x000D_
millimeters from the seventh day of sowing until the fourteenth day, it was found that_x000D_
the essential oil of lemon grass at the concentrations of 0.1, 0.5, 1, 1.5 and_x000D_
2 μL / mL completely inhibit the growth of the fungi under study. Concluding that the_x000D_
essential oil is a strong growth inhibitor of M. canis and T. rubrum.
Keywords
Aceite esencial, hierbaluisa, Microsporum canis