Efecto genotóxico de la Tartrazina a diferentes concentraciones en linfocitos de Mus musculus Balb-c evaluado mediante el test de micronúcleos
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Date
2018
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Universidad Nacional de Trujillo
Abstract
Los colorantes artificiales son ampliamente usados en la industria alimentaria; sin embargo, algunos de ellos son regulados por su posible efecto negativo en la salud humana, siendo el caso del colorante azoico tartrazina cuyo efecto tóxico ha generado discrepancias. Por ello, el objetivo del presente trabajo es determinar el efecto genotóxico de la Tartrazina a diferentes concentraciones en linfocitos de Mus musculus Balb-c evaluados mediante el test de micronúcleos. Para el efecto, se consideraron cinco grupos experimentales: control negativo (Suero fisiológico), control positivo (Dicromato de potasio 25mg/Kg) y Tartrazina a tres concentraciones (0.06 mg/ml, 0.6mg/ml y 6mg/ml), cada uno constituidos por cinco individuos de M. musculus Balb-c, a los cuales se les administró 0.5ml de cada solución resultando en dosis de 25 mg/kg de peso corporal al grupo control positivo (por única vez) y Tartrazina a dosis de 0.75 7.5 y 75 mg/kg de peso corporal. Cada 24 horas se obtuvo una gota de sangre de la cola y se realizó el frotis, secado y coloración con Wright, durante 7 días. Posteriormente, se realizó el conteo de 1000 linfocitos por individuo en todos los tratamientos. Los resultados mostraron dos grupos estadísticamente diferentes: el primer grupo, constituido por Suero fisiológico (control negativo) y Tartrazina a la concentración mínima 0.06mg/ml (dosis 0.75mg/kg de peso corporal); el segundo grupo, constituido por Dicromato de potasio a una dosis de 25mg/kg de peso corporal (control positivo) y Tartrazina a las concentraciones de 0.6 y 6 mg/ml (a dosis 7.5 y 75mg/kg de peso corporal). Se concluyó que la Tartrazina presenta efecto genotóxico e incrementa el número de linfocitos micronucleados a partir de las concentraciones 0.6 y 6 mg/ml.
Artificial dyes are widely used in the food industry; However, some of them are regulated by their possible negative effect on human health, being the case of the azo dye Tartrazine whose toxic effect has generated discrepancies. Therefore, the objective of this research is to determine the genotoxic effect of Tartrazine at different concentrations in Mus musculus Balb-c lymphocytes evaluated by the micronucleus test. For this purpose, five experimental groups were considered: negative control (physiological serum), positive control (potassium dichromate 25 mg / kg) and Tartrazine at three concentrations (0.06 mg / ml, 0.6 mg / ml and 6 mg / ml), each of them consisted of five individuals of M. musculus Balb-c, to which 0.5ml of each solution was administered resulting in a dose of 25 mg / kg of body weight to the positive control group (one time) and Tartrazine at a dose of 0.75 7.5 and 75 mg / kg of body weight. Every 24 hours a drop of blood was obtained from the tail and smeared, dried and stained with Wright dye for 7 days. Subsequently, the count of 1000 lymphocytes per individual was performed in all treatments. The results showed two statistically different groups: the first group, constituted by physiological serum (negative control) and Tartrazine at the minimum concentration 0.06mg / ml (dose 0.75mg / kg of body weight); the second group, consisting of potassium dichromate at a dose of 25 mg / kg body weight (positive control) and tartrazine at concentrations of 0.6 and 6 mg / ml (at doses 7.5 and 75 mg / kg body weight). It was concluded that Tartrazine has a genotoxic effect and increases the number of micronucleated lymphocytes from concentrations of 0.6 and 6 mg / ml.
Artificial dyes are widely used in the food industry; However, some of them are regulated by their possible negative effect on human health, being the case of the azo dye Tartrazine whose toxic effect has generated discrepancies. Therefore, the objective of this research is to determine the genotoxic effect of Tartrazine at different concentrations in Mus musculus Balb-c lymphocytes evaluated by the micronucleus test. For this purpose, five experimental groups were considered: negative control (physiological serum), positive control (potassium dichromate 25 mg / kg) and Tartrazine at three concentrations (0.06 mg / ml, 0.6 mg / ml and 6 mg / ml), each of them consisted of five individuals of M. musculus Balb-c, to which 0.5ml of each solution was administered resulting in a dose of 25 mg / kg of body weight to the positive control group (one time) and Tartrazine at a dose of 0.75 7.5 and 75 mg / kg of body weight. Every 24 hours a drop of blood was obtained from the tail and smeared, dried and stained with Wright dye for 7 days. Subsequently, the count of 1000 lymphocytes per individual was performed in all treatments. The results showed two statistically different groups: the first group, constituted by physiological serum (negative control) and Tartrazine at the minimum concentration 0.06mg / ml (dose 0.75mg / kg of body weight); the second group, consisting of potassium dichromate at a dose of 25 mg / kg body weight (positive control) and tartrazine at concentrations of 0.6 and 6 mg / ml (at doses 7.5 and 75 mg / kg body weight). It was concluded that Tartrazine has a genotoxic effect and increases the number of micronucleated lymphocytes from concentrations of 0.6 and 6 mg / ml.
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Keywords
Micronúcleos, Tartrazina, linfocitos, biomonitoreo citogenético, efecto genotóxico.