Formulación y caracterización de una nanoemulsión con actividad antioxidante
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente trabajo de investigación tuvo como finalidad, formular, elaborar y caracterizar 5 nanoemulsiones cosméticas de vitamina E con poder antioxidante, para combatir las enfermedades de la piel, retardando el envejecimiento, previniendo la oxidación de ácidos grasos insaturados y proteínas, otorgando propiedades nutritivas y regenerativas a la piel. El procedimiento se llevará a cabo siguiendo los protocolos establecidos por el método de desplazamiento de solvente. Se formularon 5 tipos de nanoemulsiones de vitamina E, a distintas concentraciones variando el peso del principio activo y el emulsificante, se rotavaporó para eliminar el exceso de solvente, seguido se caracterizó en el equipo de dispersión dinámica de luz (DLS) donde se obtuvo resultados en función del tamaño de gota, potencial zeta, índice de polidispersión, Se determinó la capacidad antioxidante de nanoemulsiones, mediante el método del radical libre 2,2-difenil-1-picrilhidracilo (DPPH), donde se utilizó 0.1 mL de nano emulsión de cada muestra, adicionando 2.9 mL de la solución de DPPH preparada, teniéndolo en oscuridad todo el proceso, se llevó 20 minutos a sonicación. se registró la medida de absorbancia a 517 nm, este proceso de lectura se realizó cada 7 días evaluando el poder antioxidante, teniendo como resultado el promedio de las lecturas de las 3 semanas, la primera muestra tiene un 10,23%, la segunda muestra tiene 20.79%, la tercera muestra tiene 16.88%, la cuarta muestra tiene 19.13% y la quinta muestra tiene 17.73% de capacidad antioxidante. Se concluye que las nanoemulsiones tienen las características organolépticas, físicas y químicas para poder denominarse como tales, además de poseer un poder antioxidante que varía por la concentración de principio activo.
ABSTRACT The purpose of this research work was to formulate, produce and characterize 5 cosmetic nanoemulsions of vitamin E with antioxidant power, to combat skin diseases, delaying aging, preventing the oxidation of unsaturated fatty acids and proteins, providing nutritional and regenerative properties to the skin. The procedure will be carried out following the protocols established by the solvent displacement method. Five types of vitamin E nanoemulsions were formulated at different concentrations by varying the weight of the active ingredient and the emulsifier, a rotary evaporation was performed to remove excess solvent, then it was characterized in the dynamic light scattering (DLS) equipment where results were obtained based on the droplet size, zeta potential, polydispersity index, The antioxidant capacity of nanoemulsions was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method, where 0.1 mL of nano emulsion from each sample was used, adding 2.9 mL of the prepared DPPH solution, keeping it in the dark throughout the process, sonication was carried out for 20 minutes. The absorbance measurement was recorded at 517 nm. This reading process was carried out every 7 days, evaluating the antioxidant power, resulting in the average of the readings of the 3 weeks. The first sample has 10.23%, the second sample has 20.79%, the third sample has 16.88%, the fourth sample has 19.13% and the fifth sample has 17.73% of antioxidant capacity. It is concluded that nanoemulsions have the organoleptic, physical and chemical characteristics to be called as such, in addition to having an antioxidant power that varies by the concentration of active ingredient.
ABSTRACT The purpose of this research work was to formulate, produce and characterize 5 cosmetic nanoemulsions of vitamin E with antioxidant power, to combat skin diseases, delaying aging, preventing the oxidation of unsaturated fatty acids and proteins, providing nutritional and regenerative properties to the skin. The procedure will be carried out following the protocols established by the solvent displacement method. Five types of vitamin E nanoemulsions were formulated at different concentrations by varying the weight of the active ingredient and the emulsifier, a rotary evaporation was performed to remove excess solvent, then it was characterized in the dynamic light scattering (DLS) equipment where results were obtained based on the droplet size, zeta potential, polydispersity index, The antioxidant capacity of nanoemulsions was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method, where 0.1 mL of nano emulsion from each sample was used, adding 2.9 mL of the prepared DPPH solution, keeping it in the dark throughout the process, sonication was carried out for 20 minutes. The absorbance measurement was recorded at 517 nm. This reading process was carried out every 7 days, evaluating the antioxidant power, resulting in the average of the readings of the 3 weeks. The first sample has 10.23%, the second sample has 20.79%, the third sample has 16.88%, the fourth sample has 19.13% and the fifth sample has 17.73% of antioxidant capacity. It is concluded that nanoemulsions have the organoleptic, physical and chemical characteristics to be called as such, in addition to having an antioxidant power that varies by the concentration of active ingredient.
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Keywords
Nanoemulsión. Vitamina E. Antioxidante.