Actividad de las vainas de Moringa oleífera L. en la hiperglucemia inducida en Rattus norvegicus cepa Holtzman
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Date
2022
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Universidad Nacional de Trujillo
Abstract
El objetivo del presente trabajo de investigación, fue determinar la actividad de las vainas de Moringa oleífera L. en la hiperglucemia inducida en Rattus norvegicus cepa Holtzman. Se utilizaron 20 especímenes de 2 meses y medio de edad, distribuidos al azar en 4 grupos de estudio; blanco, control, experimental y estándar, a los cuales se midió la glicemia basal, al grupo blanco se administró 5 mL/kg de agua hervida fría, al grupo control se administró una sobrecarga de glucosa y al grupo experimental se administró 250 mg/kg p.c del extracto de M. oleífera y luego la sobrecarga de glucosa, al grupo estándar se le administró 150 mg/kg p.c de metformina y luego la sobrecarga de glucosa. Todas las administraciones fueron vía oral. Después se midió la glucosa sanguínea a los 30, 60, 90 y 120 minutos respectivamente para los 4 grupos de estudio. Con los resultados se evidencia que en el grupo experimental hubo una reducción significativa de los niveles de glucosa en sangre de 83.80 ± 1.30 mg/dL comparado con el grupo control que fue de 100.4 ± 6.11 mg/dL y el grupo estándar que fue 98.6 ± 8.93 mg/dL. En el extracto acuoso de las vainas de Moringa oleífera L. se identificó flavonoides, saponinas, azúcares reductores, aminoácidos y compuestos fenólicos. Se concluye que el extracto de las vainas de Moringa oleífera L. administrado antes de la sobrecarga de glucosa oral, como preventivo, redujo los valores de glicemia de Rattus norvegicus cepa Holtzman.
ABSTRACT The objective of this research work was to determine the activity of Moringa oleifera L. pods in induced hyperglycemia in Rattus norvegicus Holtzman strain. Twenty specimens of 2 and a half months of age were used, randomly distributed in 4 study groups; white, control, experimental and standard, to which basal glycemia was measured, the white group was given 5 mL/kg of cold boiled water, the control group was given a glucose overload and the experimental group was given 250 mg/kg p.c of the extract of M. oleífera and then the glucose load, the standard group was administered 150 mg/kg p.c of metformin and then the glucose load. All administrations were orally. Blood glucose was then measured at 30, 60, 90, and 120 minutes, respectively, for the 4 study groups. With the results, it is evident that in the experimental group there was a significant reduction in blood glucose levels of 83.80 ± 1.30 mg/dL compared to the control group that was 100.4 ± 6.11 mg/dL and the standard group that was 98.6 ± 8.93mg/dL. Flavonoids, saponins, reducing sugars, amino acids and phenolic compounds were identified in the aqueous extract of Moringa oleifera L. pods. It is concluded that the extract of the pods of Moringa oleifera L. administered before oral glucose overload, as a preventive, reduced the glycemia values of Rattus norvegicus Holtzman strain.
ABSTRACT The objective of this research work was to determine the activity of Moringa oleifera L. pods in induced hyperglycemia in Rattus norvegicus Holtzman strain. Twenty specimens of 2 and a half months of age were used, randomly distributed in 4 study groups; white, control, experimental and standard, to which basal glycemia was measured, the white group was given 5 mL/kg of cold boiled water, the control group was given a glucose overload and the experimental group was given 250 mg/kg p.c of the extract of M. oleífera and then the glucose load, the standard group was administered 150 mg/kg p.c of metformin and then the glucose load. All administrations were orally. Blood glucose was then measured at 30, 60, 90, and 120 minutes, respectively, for the 4 study groups. With the results, it is evident that in the experimental group there was a significant reduction in blood glucose levels of 83.80 ± 1.30 mg/dL compared to the control group that was 100.4 ± 6.11 mg/dL and the standard group that was 98.6 ± 8.93mg/dL. Flavonoids, saponins, reducing sugars, amino acids and phenolic compounds were identified in the aqueous extract of Moringa oleifera L. pods. It is concluded that the extract of the pods of Moringa oleifera L. administered before oral glucose overload, as a preventive, reduced the glycemia values of Rattus norvegicus Holtzman strain.
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Keywords
Moringa oleífera L. Glicemia. Tolerancia a la glucosa. Diabetes mellitus