Marcadores Químicos de Desmodium molliculum (Kunth) DC. Dispensado en medicina complementaria de ESSALUD
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Date
2023
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Universidad Nacional de Trujillo
Abstract
La especie vegetal Desmodium molliculum (Kunth) DC. es dispensada en Medicina Complementaria de EsSalud y es utilizada como antiinflamatorio. La muestra vegetal de D. molliculum fue identificada en el Herbarium Truxillense. Se seleccionaron tres lotes diferentes de D. molliculum y se prepararon extractos metanólicos por el método de maceración con movimiento constante por 24 horas. Luego, se analizaron por cromatografía líquida y se detectaron picos mayoritarios a 254 y 330 nm. Se identificó tentativamente la estructura química del pico mayoritario a través del equipo de UHPLC-MS/MS. Se obtuvieron 10 metabolitos con el extracto metanólico y se les nombró con las letras desde la “A” hasta la “J”. Se observó que en los tres lotes analizados los picos y áreas fueron estadísticamente idénticos. El pico “D” tuvo la mayor área en los cromatogramas en ambas longitudes de onda. Se identificó que la señal “D” correspondería a la molécula vitexina de m/z 431. Se concluye que los perfiles cromatográficos de los tres lotes de Desmodium molliculum (Kunth) DC. mostraron 10 compuestos, siendo la vitexina un marcador químico del género Desmodium.
The plant species Desmodium molliculum (Kunth) DC. is dispensed in Complementary Medicine of EsSalud and it is used as an anti-inflammatory. The vegetal sample of D. molliculum was identified in the Herbarium Truxillense. Three different batches of D. molliculum were selected and methanolic extracts were made by the maceration method with constant movement for 24 hours. Then, they were analyzed by liquid chromatography and major peaks were detected at 254 and 330 nm. The chemical structure of the majority peak was tentatively identified by UHPLC-MS/MS. Ten secondary metabolites were obtained with the methanolic extract and named with the letters “A” through “J”. It was observed that in the three batches analyzed the peaks and areas were statistically identical. Peak “D” had the largest area in the chromatograms at both absorbances. It was tentatively identified that the “D” signal would correspond to the m/z 431 Vitexin molecule. It is concluded that the chromatographic profiles of the three batches of Desmodium molliculum (Kunth) DC. showed 10 compounds, vitexin being a chemical marker of the genus Desmodium.
The plant species Desmodium molliculum (Kunth) DC. is dispensed in Complementary Medicine of EsSalud and it is used as an anti-inflammatory. The vegetal sample of D. molliculum was identified in the Herbarium Truxillense. Three different batches of D. molliculum were selected and methanolic extracts were made by the maceration method with constant movement for 24 hours. Then, they were analyzed by liquid chromatography and major peaks were detected at 254 and 330 nm. The chemical structure of the majority peak was tentatively identified by UHPLC-MS/MS. Ten secondary metabolites were obtained with the methanolic extract and named with the letters “A” through “J”. It was observed that in the three batches analyzed the peaks and areas were statistically identical. Peak “D” had the largest area in the chromatograms at both absorbances. It was tentatively identified that the “D” signal would correspond to the m/z 431 Vitexin molecule. It is concluded that the chromatographic profiles of the three batches of Desmodium molliculum (Kunth) DC. showed 10 compounds, vitexin being a chemical marker of the genus Desmodium.
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Keywords
Desmodium molliculum, Vitexina, Marcador químico, CLAR, Espectrometría de masas