Evaluación in vitro del efecto de aceite esencial de Cymbopogon citratus (DC.) Stapf. “hierba luisa”, sobre el crecimiento micelial de Colletotrichum gloeosporioides (Penz.) Penz. y Sacc.
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Date
2024
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Universidad Nacional de Trujillo
Abstract
El presente trabajo de investigación tiene como objetivo evaluar el efecto inhibitorio del aceite esencial de Cymbopogon citratus (DC.) Stapf. “hierba luisa” sobre el crecimiento micelial de Colletotrichum gloeosporioides (Penz.) Penz. y Sacc., se recolectaron plantas sanas del centro poblado de Buena Vista, Provincia de Virú, Región La Libertad. Se seleccionaron las hojas integras las cuales fueron lavadas con agua destilada estéril, desinfectadas en hipoclorito de sodio al 2% y secadas a temperatura ambiente. La obtención del aceite esencial se llevó a cabo mediante el método de destilación por arrastre de vapor de agua. Los cultivos puros de los hongos fueron proporcionados por el Laboratorio de Tecnología Enzimática y Productos Naturales de la Universidad Nacional de Trujillo. Se evaluó el efecto inhibitorio utilizando el método propuesto por Vincet bajo un diseño completamente al azar. Se preparó las diluciones de aceite esencial con Tween en las concentraciones de 1, 2, 5 y 10 μL/mL, un control positivo con carbendazim al 0,5 cc/L y un control negativo con agua destilada, cada uno con cinco repeticiones; asimismo se determinó el porcentaje de inhibición, análisis de varianza y la prueba de Tukey. Los resultados obtenidos mostraron inhibición en todas las concentraciones utilizadas (1, 2, 5 y 10 μL/mL), sobre C. gloeosporioides (Penz.) Penz. y Sacc., in vitro. Sin diferencias significativas en estos tratamientos.
The present research work aims to evaluate the inhibitory effect of the essential oil of Cymbopogon citratus (DC.) Stapf. “hierva luisa” on the mycelial growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., Healthy plants were collected from the town of Buena Vista, Virú Province, La Libertad Region. The whole leaves were selected, which were washed with sterile distilled water, disinfected in 2% sodium hypochlorite and dried at room temperature. The essential oil was obtained by the steam distillation method. The pure cultures of the fungi were provided by the Laboratory of Enzyme Technology and Natural Products of the National University of Trujillo. The inhibitory effect was evaluated using the method proposed by Vincet under a completely randomized design. Essential oil dilutions were prepared with Tween at concentrations of 1, 2, 5 and 10 μL/mL, a positive control with carbendazim at 0.5 cc/L and a negative control with distilled water, each with five replicates; the percentage of inhibition was also determined, analysis of variance and the Tukey test. The results obtained showed inhibition at all concentrations used (1, 2, 5 and 10 μL/mL), on C. gloeosporioides (Penz.) Penz. and Sacc., in vitro. No significant differences were found in these treatments.
The present research work aims to evaluate the inhibitory effect of the essential oil of Cymbopogon citratus (DC.) Stapf. “hierva luisa” on the mycelial growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., Healthy plants were collected from the town of Buena Vista, Virú Province, La Libertad Region. The whole leaves were selected, which were washed with sterile distilled water, disinfected in 2% sodium hypochlorite and dried at room temperature. The essential oil was obtained by the steam distillation method. The pure cultures of the fungi were provided by the Laboratory of Enzyme Technology and Natural Products of the National University of Trujillo. The inhibitory effect was evaluated using the method proposed by Vincet under a completely randomized design. Essential oil dilutions were prepared with Tween at concentrations of 1, 2, 5 and 10 μL/mL, a positive control with carbendazim at 0.5 cc/L and a negative control with distilled water, each with five replicates; the percentage of inhibition was also determined, analysis of variance and the Tukey test. The results obtained showed inhibition at all concentrations used (1, 2, 5 and 10 μL/mL), on C. gloeosporioides (Penz.) Penz. and Sacc., in vitro. No significant differences were found in these treatments.
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Keywords
Efecto inhibitorio, Cymbopogon citratus, Colletotrichum gloeosporioides, aceite esencial, hierva luisa