Aislamiento y purificación de mangiferina de Gentianella nitida (Griseb.) Fabris por cromatografía líquida semipreparativa
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Date
2021
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Universidad Nacional de Trujillo
Abstract
La investigación tuvo como objetivo aislar y purificar mangiferina de Gentianella nitida (Griseb.) Fabris por cromatografía líquida semipreparativa. El aislamiento se realizó a partir de muestra seca con metanol, por baño de ultrasonido, obteniendo un extracto que al concentrarlo y enfriarlo a 2 °C produjo un precipitado con características similares a los cristales de mangiferina. Para la identificación de mangiferina se comparó el perfil cromatográfico a 254 nm con un estándar. La purificación se realizó mediante la cromatografía líquida semipreparativa, con una columna de octadecilsilano y fase móvil agua:acetonitrilo a 3 mL/min por 45'. Para la cuantificación se obtuvo una curva de calibración con el estándar de mangiferina en un rango de 1 a 160 ppm por cromatografía liquida de alta resolución. Se determinó el 5,27 % de rendimiento del precipitado obtenido del aislamiento de mangiferina a partir de la muestra seca. La recuperación de mangiferina purificada fue de 458,30 ± 11,21 mg por cada gramo de precipitado obtenido del aislamiento. Se concluye que aplicando la técnica de cromatografía líquida semipreparativa se obtiene 2,41 % de mangiferina a partir de la muestra seca de Gentianella nitida (Griseb.) Fabris.
ABSTRACT The research aimed to isolate and purify mangiferin from Gentianella nitida (Griseb.) Fabris by semi-preparative liquid chromatography. The isolation was carried out from a dry sample with methanol, by ultrasound bath, obtaining an extract that when concentrated and cooled to 2 °C produced a precipitate with characteristics similar to mangiferin crystals. For the identification of mangiferin, the chromatographic profile at 254 nm was compared with a standard. Purification was carried out by means of semi-preparative liquid chromatography, with an octadecylsilane column and water:acetonitrile mobile phase at 3 ml/min for 45'. To quantify this product, a calibration curve of mangiferin standard was obtained, in a range from 1 to 160 ppm by high-resolution liquid chromatography. The 5.27% yield of the precipitate obtained from the isolation of mangiferin from the dry sample was determined. The recovery of purified mangiferin was 458.30 ± 11.21 mg for each gram of precipitate obtained from the isolation. It is concluded that applying the semi-preparative liquid chromatography technique, 2.41% of mangiferin is obtained from the dry sample of Gentianella nitida (Griseb.) Fabris.
ABSTRACT The research aimed to isolate and purify mangiferin from Gentianella nitida (Griseb.) Fabris by semi-preparative liquid chromatography. The isolation was carried out from a dry sample with methanol, by ultrasound bath, obtaining an extract that when concentrated and cooled to 2 °C produced a precipitate with characteristics similar to mangiferin crystals. For the identification of mangiferin, the chromatographic profile at 254 nm was compared with a standard. Purification was carried out by means of semi-preparative liquid chromatography, with an octadecylsilane column and water:acetonitrile mobile phase at 3 ml/min for 45'. To quantify this product, a calibration curve of mangiferin standard was obtained, in a range from 1 to 160 ppm by high-resolution liquid chromatography. The 5.27% yield of the precipitate obtained from the isolation of mangiferin from the dry sample was determined. The recovery of purified mangiferin was 458.30 ± 11.21 mg for each gram of precipitate obtained from the isolation. It is concluded that applying the semi-preparative liquid chromatography technique, 2.41% of mangiferin is obtained from the dry sample of Gentianella nitida (Griseb.) Fabris.
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Keywords
Cromatografía líquida