Efecto in vitro de Beauveria bassiana Bb-UNT01 sobre larvas de Diatraea saccharalis
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Se evaluó el efecto in vitro de Beauveria bassiana Bb-UNT01 sobre larvas de Diatraea saccharalis. Para esto, se obtuvieron los especímenes en estadios larvarios diferentes de D. saccharalis y se realizó el acondicionamiento de las larvas. Luego se realizaron subcultivos de B. bassiana y se propagaron en frascos planos conteniendo Agar Sabouraud. Posteriormente se emplearon cuatro tratamientos con tres repeticiones cada uno, a cada tratamiento se les inoculó por aspersión las concentraciones estandarizadas de 104, 105 y 106 conidios/mL de B. bassiana, mientras al tratamiento control se inoculó por aspersión la suspensión de Tween 80 al 0.1 %. Luego a las larvas inoculadas fueron colocados en recipientes de plásticos acondicionadas con segmentos de tallos de maíz y caña de azúcar. Todos los tratamientos se mantuvieron a 26°C y con una humedad relativa de 70%. Después de la inoculación, las larvas fueron evaluadas durante 15 días y presentaron síntomas como lentitud en movimiento, pérdida de apetito, alteración de color del tegumento y muerte. Las larvas muertas fueron transferidas a una cámara húmeda hasta la aparición del micelio y luego se procedió a observar al microscopio. Los resultados mostraron que a la concentración de 104 conidios/mL de B. bassiana fue de 73.3%, seguido a la concentración de 105 conidios/mL fue de 53.3 % y a la concentración de 106 conidios/mL fue de 50.0 %. Por otra parte, en el tratamiento control, en el cual no se inoculó conidios de B. bassiana, presentaron un porcentaje de supervivencia de larvas de D. saccharalis del 100%. Por lo tanto, existe una diferencia significativa en el tratamiento 104 con/mL y son estadísticamente iguales los tratamientos de 105 y 106 con/mL lo cual demuestra que las concentraciones empleadas afectan a la supervivencia de las larvas de D. saccharalis.
The in vitro effect of Beauveria bassiana Bb-UNT01 on Diatraea saccharalis larvae was evaluated. For this purpose, specimens were obtained in larval stages of D. saccharalis and larval conditioning was performed. Subcultures of B. bassiana were then subcultured and propagated in flat flasks containing Sabouraud Agar. Subsequently, four treatments with three replicates each were used, each treatment was spray inoculated with standardized concentrations of 104, 105 and 106 conidia/mL of B. bassiana, while the control treatment was spray inoculated with 0.1% Tween 80 suspension. The inoculated larvae were then placed in plastic containers conditioned with segments of corn and sugarcane stalks. All treatments were maintained at 26°C and a relative humidity of 70%. After inoculation, the larvae were evaluated for 15 days and presented symptoms such as slow movement, loss of appetite, altered color of the integument and death. The dead larvae were transferred to a humid chamber until the mycelium appeared and then they were observed under the microscope. The results showed that at the concentration of 104 conidia/mL of B. bassiana was 73.3%, followed at the concentration of 105 conidia/mL was 53.3% and at the concentration of 106 conidia/mL was 50.0%. On the other hand, in the control treatment, in which B. bassiana conidia were not inoculated, the percentage of survival of D. saccharalis larvae was 100%. Therefore, there is a significant difference in the 104 con/mL treatment and the 105 and 106 con/mL treatments are statistically equal, which shows that the concentrations used affect the survival of D. saccharalis larvae.
The in vitro effect of Beauveria bassiana Bb-UNT01 on Diatraea saccharalis larvae was evaluated. For this purpose, specimens were obtained in larval stages of D. saccharalis and larval conditioning was performed. Subcultures of B. bassiana were then subcultured and propagated in flat flasks containing Sabouraud Agar. Subsequently, four treatments with three replicates each were used, each treatment was spray inoculated with standardized concentrations of 104, 105 and 106 conidia/mL of B. bassiana, while the control treatment was spray inoculated with 0.1% Tween 80 suspension. The inoculated larvae were then placed in plastic containers conditioned with segments of corn and sugarcane stalks. All treatments were maintained at 26°C and a relative humidity of 70%. After inoculation, the larvae were evaluated for 15 days and presented symptoms such as slow movement, loss of appetite, altered color of the integument and death. The dead larvae were transferred to a humid chamber until the mycelium appeared and then they were observed under the microscope. The results showed that at the concentration of 104 conidia/mL of B. bassiana was 73.3%, followed at the concentration of 105 conidia/mL was 53.3% and at the concentration of 106 conidia/mL was 50.0%. On the other hand, in the control treatment, in which B. bassiana conidia were not inoculated, the percentage of survival of D. saccharalis larvae was 100%. Therefore, there is a significant difference in the 104 con/mL treatment and the 105 and 106 con/mL treatments are statistically equal, which shows that the concentrations used affect the survival of D. saccharalis larvae.
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