Efecto in vitro del aceite esencial de las hojas de Mentha spicata sobre el crecimiento de Microsporum gypseum
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Date
2024
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Universidad Nacional de Trujillo
Abstract
Se evaluó el efecto del aceite esencial de las hojas de Mentha spicata sobre el crecimiento de Microsporum gypseum in vitro. Se trabajó con el vegetal procedente de los cultivos agrícolas de Pedregal, Distrito de Simbal, Provincia de Trujillo, La Libertad; la obtención del aceite esencial se realizó mediante el método de hidrodestilación en equipo Clevenger. Para evaluar su efecto se utilizó la técnica de incorporación del aceite en el medio de cultivo Agar Dextrosa Sabouraud, agregando la cantidad adecuada del aceite esencial hasta obtener las concentraciones de 400, 500, 600 y 700 μg/mL y como control negativo el medio de cultivo. Luego, se sembró por puntura central un fragmento de micelio de M. gypseum, se incubó a 25°C por una semana, se realizaron las lecturas del crecimiento radial en milímetros de diámetro. Se concluye que el aceite esencial a las concentraciones evaluadas inhibe el crecimiento de M. gypseum, en proporción directa a la concentración, es decir, a mayor concentración mayor inhibición del crecimiento radial. Resultando una alternativa prometedora para el tratamiento contra estos patógenos, gracias a sus componentes bioactivos
The effect of the essential oil of Mentha spicata leaves on the growth of Microsporum gypseum in vitro was evaluated. We worked with the vegetable from the agricultural crops of Pedregal, District of Simbal, Province of Trujillo, La Libertad; obtaining the essential oil using the hydrodistillation method in Clevenger equipment. To evaluate its effect, the technique of incorporating the oil into the Sabouraud Dextrose Agar culture medium was used, adding the appropriate amount of the essential oil until obtaining concentrations of 400, 500, 600 and 700 μg/mL and as a negative control the medium of crop. Then, a fragment of M. gypseum mycelium was planted by central puncture, incubated at 25°C for a week, readings of radial growth in millimeters of diameter were made. It is concluded that the essential oil at the concentrations evaluated inhibits the growth of M. gypseum, in direct proportion to the concentration, that is, the higher the concentration, the greater the inhibition of radial growth. Resulting in a promising alternative for the treatment against these pathogens, thanks to its bioactive components.
The effect of the essential oil of Mentha spicata leaves on the growth of Microsporum gypseum in vitro was evaluated. We worked with the vegetable from the agricultural crops of Pedregal, District of Simbal, Province of Trujillo, La Libertad; obtaining the essential oil using the hydrodistillation method in Clevenger equipment. To evaluate its effect, the technique of incorporating the oil into the Sabouraud Dextrose Agar culture medium was used, adding the appropriate amount of the essential oil until obtaining concentrations of 400, 500, 600 and 700 μg/mL and as a negative control the medium of crop. Then, a fragment of M. gypseum mycelium was planted by central puncture, incubated at 25°C for a week, readings of radial growth in millimeters of diameter were made. It is concluded that the essential oil at the concentrations evaluated inhibits the growth of M. gypseum, in direct proportion to the concentration, that is, the higher the concentration, the greater the inhibition of radial growth. Resulting in a promising alternative for the treatment against these pathogens, thanks to its bioactive components.
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Keywords
Inhibición del crecimiento fúngico