Puntuación de micronúcleos en mucosa bucal de pacientes con cáncer colorrectal expuestos al sevoflurano
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Date
2023-02-06
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Universidad Nacional de Trujillo
Abstract
El objetivo del proyecto de investigación es determinar las diferencias en la puntuación de micronúcleos en mucosa bucal de pacientes con cáncer colorrectal antes y después de la exposición al sevoflurano. Se utilizará como muestra las células de mucosa bucal de pacientes con adenocarcinoma colorrectal operados bajo anestesia general con mantenimiento de solo 1 CAM de sevoflurano en el HVLE entre enero del 2023 y abril del 2023. Las células se fijarán con Carnoy, se teñirán con Giemsa y se dejarán secar al medio ambiente. Se utilizará un microscopio óptico CARL ZEISS PRIMO STAR a 1000X de aumento y aceite de inmersión para estimar las puntuaciones de micronúcleos (frecuencia de micronúcleos y frecuencia de células micronucleadas) e identificar las variaciones en tamaño y número de los micronúcleos, antes y después de la exposición al sevoflurano. Posteriormente se establecerán la diferencia significativa en las puntuaciones de micronúcleos.
Abstract The research project aims to determine the differences in the micronucleus score in oral mucosa of patients with colorectal cancer before and after exposure to sevoflurane. Oral mucosa cells from patients with colorectal adenocarcinoma operated under general anesthesia with maintenance of only 1 MAC of sevoflurane in the HVLE between January 2023 and April 2023 will be used as a sample. Cells will be fixed with Carnoy, stained with Giemsa and allowed to air dry. A CARL ZEISS PRIMO STAR optical microscope at 1000X magnification and immersion oil will be used to estimate micronucleus scores (frequency of micronuclei and frequency of micronucleated cells) and identify variations in size and number of micronuclei, before and after exposure to sevoflurane. Subsequently, the significant difference in the micronucleus scores will be established.
Abstract The research project aims to determine the differences in the micronucleus score in oral mucosa of patients with colorectal cancer before and after exposure to sevoflurane. Oral mucosa cells from patients with colorectal adenocarcinoma operated under general anesthesia with maintenance of only 1 MAC of sevoflurane in the HVLE between January 2023 and April 2023 will be used as a sample. Cells will be fixed with Carnoy, stained with Giemsa and allowed to air dry. A CARL ZEISS PRIMO STAR optical microscope at 1000X magnification and immersion oil will be used to estimate micronucleus scores (frequency of micronuclei and frequency of micronucleated cells) and identify variations in size and number of micronuclei, before and after exposure to sevoflurane. Subsequently, the significant difference in the micronucleus scores will be established.
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Keywords
Sevoflurano, Neoplasias Colorrectales, Pruebas de micronúcleos