Efecto de la inoculación de bacterias diazótrofas endófitas en el crecimiento de plántulas de Asparagus officinalis L. var. UC 157
No Thumbnail Available
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Trujillo
Abstract
El presente estudio tuvo como objetivo evaluar el efecto de la inoculación de bacterias diazótrofas endófitas en el crecimiento de plántulas de Asparagus officinalis var. UC 157, apuntando al desarrollo y aplicación de estrategias que permitan el uso de técnicas sostenibles para el manejo nutricional del espárrago. Tres cepas de bacterias endófitas de la colección del Laboratorio de Análisis de Suelos y Foliares (LASFB), aisladas de diferentes hospederos se inocularon individualmente y en mezcla en semillas de espárrago a una densidad poblacional de 3.5x108 UFC.ml. El experimento, conducido bajo condiciones de casa malla en DBCA, mostró efectos estimuladores de las cepas LASFB P086 (Klebsiella sp) y LASFB 1430 (no identificada) sobre el cultivo, con aumentos en el número de brotes (106% y 94%), materia fresca (52% y 54%), longitud de raíces (22% y 19%), número de raíces (78% y 72%) y materia seca (32% y 28%); mientras que en altura de la plántula y la materia fresca radicular no mostraron diferencias estadísticas significativas. La cepa LASFB 292 (Stenotrophomonas sp) y el tratamiento mezcla Mix 3 no mostraron diferencia estadística respecto al testigo en ninguna de las variables evaluadas. Todos los datos expuestos fueron procesados mediante la prueba de medias de Tuckey al 5 % de significación.
ABSTRACT The objective of the present study was to evaluate the effect of the inoculation of endophytic diazotrophic bacteria on the growth of seedlings of Asparagus officinalis var. UC 157, aiming at the development and application of strategies that allow the use of sustainable techniques for the nutritional management of asparagus. Three strains of endophytic bacteria from the Soil and Foliar Analysis Laboratory (LASFB) collection, isolated from different hosts, were inoculated individually and mixed into asparagus seeds at a population density of 3.5x108 CFU.ml. The experiment, conducted under domestic mesh conditions in DBCA, showed stimulatory effects of strains LASFB P086 (Klebsiella sp) and LASFB 1430 (unidentified) on the crop, with increases in the number of shoots (106% and 94%), fresh (52% and 54%), root length (22% and 19%), number of roots (78% and 72%) and dry matter (32% and 28%); while in height of the seedling and fresh root matter did not show significant statistical differences. The strain LASFB 292 (Stenotrophomonas sp) and the Mix 3 mixed treatment showed no statistical difference with respect to the control in any of the variables evaluated. All the exposed data were processed through Tuckey's means test at 5% significance.
ABSTRACT The objective of the present study was to evaluate the effect of the inoculation of endophytic diazotrophic bacteria on the growth of seedlings of Asparagus officinalis var. UC 157, aiming at the development and application of strategies that allow the use of sustainable techniques for the nutritional management of asparagus. Three strains of endophytic bacteria from the Soil and Foliar Analysis Laboratory (LASFB) collection, isolated from different hosts, were inoculated individually and mixed into asparagus seeds at a population density of 3.5x108 CFU.ml. The experiment, conducted under domestic mesh conditions in DBCA, showed stimulatory effects of strains LASFB P086 (Klebsiella sp) and LASFB 1430 (unidentified) on the crop, with increases in the number of shoots (106% and 94%), fresh (52% and 54%), root length (22% and 19%), number of roots (78% and 72%) and dry matter (32% and 28%); while in height of the seedling and fresh root matter did not show significant statistical differences. The strain LASFB 292 (Stenotrophomonas sp) and the Mix 3 mixed treatment showed no statistical difference with respect to the control in any of the variables evaluated. All the exposed data were processed through Tuckey's means test at 5% significance.
Description
Keywords
Espárrago, Bacteria, Endófitas, Inoculación